New angucycline C-glycosides from Streptomyces sp. RI33

Our plain-English summary of New angucycline C-glycosides from Streptomyces sp. RI33, by J.Y. Ueda, M. Izumikawa, A. Mukai, et al (The Journal of Antibiotics, 2011).

The study

• Authors: J.Y. Ueda, M. Izumikawa, A. Mukai, A. Nagai, J.H. Hwang, M. Takagi, K. Shin-ya
• Journal: The Journal of Antibiotics
• Published: 2011;64(5):367-72
• Cited by: 8 articles, as counted by NIH iCite
• Read it: PubMed · Publisher

Abstract

In the course of our screening program for anticancer agents from natural sources, five new angucyclines, JBIR-90 (1), -116 (2), -91 (3), -92 (4) and -93 (5), were isolated from the fermentation broth of Streptomyces sp. RI33. The structures of 1-5 were elucidated on the basis of 1D and 2D NMR spectroscopy and MS analyses. Compounds 2-4 showed cytotoxic activities against malignant pleural mesothelioma cells at IC(50) values of 20-50 μM.

© 2011 Japan Antibiotics Research Association All rights reserved

In plain English

This was a laboratory chemistry study, not a study of people or of asbestos exposure. While screening natural sources for anticancer agents, the authors grew a bacterium, Streptomyces sp. RI33, which had been isolated from a lichen in a soil sample collected at Shuri, Okinawa Prefecture, Japan. From the culture they isolated five new compounds of the angucycline type, named JBIR-90, JBIR-116, JBIR-91, JBIR-92 and JBIR-93. They worked out the compounds' chemical structures using NMR spectroscopy and mass spectrometry. They then tested the compounds for cell-killing (cytotoxic) activity on two human cancer cell lines grown in the laboratory: ACC-MESO-1, a malignant pleural mesothelioma line, and HeLa, a cervical carcinoma line. On the mesothelioma cells, JBIR-116, JBIR-91 and JBIR-92 showed cytotoxic activity, with IC50 values of 45, 23 and 46 micromolar respectively. JBIR-90 and JBIR-93 showed no cytotoxicity against these cells at 100 micromolar. All five compounds showed cytotoxic activity against HeLa cells, with IC50 values of 18 to 75 micromolar. These were cell-culture results only.

We wrote this plain-English summary from the full article with the help of AI, and a second AI model checked each sentence against the article. It is not the authors' wording; the abstract and the article are their own account.

What the study did and found

• Design: Laboratory study: the authors isolated compounds from a bacterial culture, worked out their chemical structures, and tested them on cancer cell lines in the laboratory (in vitro). No people or animals were studied.
• Who was studied: The bacterium Streptomyces sp. RI33, isolated from a lichen in a soil sample collected at Shuri, Okinawa Prefecture, Japan. The compounds were tested on two human cancer cell lines: ACC-MESO-1 (malignant pleural mesothelioma) and HeLa (cervical carcinoma).
• Exposure: No asbestos exposure was studied. The cancer cells were treated with the five newly isolated compounds (JBIR-90, -116, -91, -92 and -93) at various concentrations for 48 hours.
• Main results:
  • Five new angucycline compounds were isolated from the Streptomyces sp. RI33 culture: JBIR-90, JBIR-116, JBIR-91, JBIR-92 and JBIR-93.
  • Against the mesothelioma cell line ACC-MESO-1, JBIR-116, JBIR-91 and JBIR-92 showed cytotoxic activity, with IC50 values of 45, 23 and 46 micromolar respectively.
  • JBIR-90 and JBIR-93 did not show cytotoxicity against ACC-MESO-1 mesothelioma cells at a concentration of 100 micromolar.
  • All five compounds showed cytotoxic activity against HeLa cervical carcinoma cells, with IC50 values of 18 to 75 micromolar.
• The authors' conclusion: The authors reported that compounds JBIR-116, JBIR-91 and JBIR-92 showed cytotoxic activity against malignant pleural mesothelioma cells at IC50 values of 20 to 50 micromolar. They drew no broader conclusion.

Topics

The National Library of Medicine indexes this study under subjects our site covers: Mesothelioma.

Medical terms in this study, explained in our Lexicon: Pleural Mesothelioma.

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